Journal: Biomaterials

Article Title: Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype

doi: 10.1016/j.biomaterials.2012.03.041

Figure Lengend Snippet: Humoral and cellular responses after vaccination with liposomes and PLGA nanoparticles. Mice (N = 4) were subcutaneously administered a single dose of 100 μg of OVA encapsulated in liposomes or PLGA nanoparticles. Mice were bled approximately every 2 weeks and analyzed for antigen-specific (A) total IgG, (B) IgG1, and (C) IgG2b titers by ELISA. At 11 weeks, splenocytes were pulsed ex vivo with OVA at 25 and 50 μg/mL and analyzed for (D) IFN-γ in the supernatant after 72 h by ELISA. Data shown are from a single experiment that was performed twice with the same results. * Indicate P value less than 0.05.

Article Snippet: Supernatant was collected and analyzed for IFN-γ secretion by ELISA (BD Pharmingen).

Techniques: Enzyme-linked Immunosorbent Assay, Ex Vivo

Journal: Biomaterials

Article Title: Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype

doi: 10.1016/j.biomaterials.2012.03.041

Figure Lengend Snippet: Post-vaccination recall of Listeria monocytogenes (LM-OVA). (A) Study timeline. Mice received a single subcutaneous administration of OVA adsorbed to aluminum hydroxide or encapsulated in liposomes or PLGA nanoparticles. At 13 weeks, mice were divided into two groups(N = 3) and received intravenous (I.V) tail vein injections with either 1 × 105 (group I) or 5 × 104 (group II) CFU of LM-OVA. At 3 days post-challenge, splenocytes from Group I mice were assayed for bacterial titers. At 7 days post-challenge, splenocytes from group II mice were stained for effector T cell surface markers and intracellular IFN-γ and analyzed by flow cytometry. (B) Humoral response at time of challenge. Serum anti-OVA IgG was measured by ELISA. * Indicate a P value less than 0.001. C) LM-OVA titers in the spleen. Three days post-LM-OVA challenge, splenocytes from Group I mice were permeabilized and plated on agar overnight. Colony forming units (CFU) were calculated and normalized by mass of the organ. Study was repeated twice with same results. *Indicates a P value less than 0.05.

Article Snippet: Supernatant was collected and analyzed for IFN-γ secretion by ELISA (BD Pharmingen).

Techniques: Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Biomaterials

Article Title: Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype

doi: 10.1016/j.biomaterials.2012.03.041

Figure Lengend Snippet: Effector-like T cell analysis. Splenocytes from group II mice (N = 3) were pulsed with SIINFEKL and (A) IFN-γ expressing CD8+ T cell population was enumerated. (B) Activated antigen-specific CD8+ CD44 + T cells were also determined using a SIINFEKL tetramer. This population in C) was analyzed for surface markers indicative of an effector-like phenotype (KLRG1hi, CD127lo, CD27lo, CD62Llo). Shaded = PBS, Black = PLGA, Red = Liposome, Blue = Alum. Study was repeated twice with same results. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Supernatant was collected and analyzed for IFN-γ secretion by ELISA (BD Pharmingen).

Techniques: Expressing